Rat IL-6 ELISA or Bioassay
shopkins at fs1.ho.man.ac.uk
Fri Mar 31 12:26:58 EST 1995
In article <3lctg2$18a at harbinger.cc.monash.edu.au>,
cyberdoc at silas.cc.monash.edu.au says...
>Could anyone help us out with some info on either:
>a RAT IL-6 ELISA kit or reagents (including hybridomas)
>or a RAT IL-6 BIOASSAY (availability of cell lines etc)
You'll have trouble identifying reagents for an ELISA (though love to
hear from anyone who has some): we've been interested in anti-rat
cytokine antibodies for ages and they are pretty thin on the ground.
Bioassay is easy though. Several line are available. We've used the B9
hybridoma (from Luciene Aarden, CLB, Amsterdam) for many years to assay
rat IL-6. It is one of the best and most sensitive cell lines for
cytokine bioassay and should be easily available. Does recognise murine
IL-4 and some neuropoietins, but not well.
Let me know if you have a problem getting hold of it.
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