Reaction/mechanism for BCIP+NBT in immunoblotting

U12201 at uicvm.uic.edu U12201 at uicvm.uic.edu
Tue Mar 28 14:23:31 EST 1995

Call Pierce Chemical at 1-800-874-3723 or E-mail PierceChem at mcimail.com
for a catalog - I am pretty sure it is in there. Anyways the BCIP is
being dephosphorylated by the Alk Phos and the resulting indol reacts
with the NBT (redox reaction). NBT can be couple to a lot of other

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