Wayne R. Baker
baker at iastate.edu
Wed May 10 22:14:13 EST 1995
In bionet.immunology, Ch. Hasse <hasse at papin.HRZ.Uni-Marburg.DE> wrote
:PMSF (Phenylmethylsulfonylfluoride) can be used for whole blood collection
:to prevent coagulation. Does anybody know how PMSF can be solved in HEPES
:without using alcohols or DMSO?
You can put it in any aqueous solution, but it won't last very long.
Hydrolysis is both acid and base catalyzed. It can be stored for
extended periods at low temp in organic solvents, like IPA, but will be
neutralized in an aqueous solution.
If you can't tolerate organics in your solution, try adding the
appropriate amount of PMSF out of the bottle. I wouldn't trust a
water-based solution of PMSF to have any effect.
Wayne Baker (baker at iastate.edu) Now, if ever, our vocal cords ought
4288 Molecular Biology Building to be played on the keyboard
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