protease inhibitors during immunolabeling

Shahram Mori smori at nmsu.edu
Wed Nov 1 01:17:17 EST 1995


User PC (blaineau at neurosens.univ-lyon1.fr) wrote:
: we realize immunogold labeling on unfixed, permeabilized muscle cells. 
: Because our incubations are very long (24 h), we need add protease 
: inhibitors to reduce ultrastructural cell damages.
: Do you know one ? At which concentration? References?
: Thank you for your assistance
: Sylvie

Try PMSF, Aprotonin, Pepstatin.
Cheers 
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Shahram Mori
Program in Molecular Biology
Department of Chemistry and Biochemistry Box 3C
New Mexico State University
Las Cruces NM
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