Monoclonal protocol ?

NEVIL J SINGH nevil at tifrvax.tifr.res.in
Tue Nov 7 12:47:42 EST 1995


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Hi,
 I am to set up my first fusion for mice hybridomas (Bcell), using SP2/0 
cells in a couple of days time.
I was wondering if any body could send me some tried & tested protocol
being regularly used in your lab.s ;...as of now I've made up a protocol 
from various ref.s & don't know if it will work. 
 ** And, how good should the antibody  titre be, before I sacrifice the 
animal ? 
 I'll be grateful for any advices/hints/suggestions..
sincerely,
- Nevil 

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