How many CD4/CD8 molecules per cell?

Keith R. Kuhn kkuhn at pennet.net
Sat Nov 11 17:53:02 EST 1995


In article <47m6rd$8bs at newsbf02.news.aol.com>,
   alussow at aol.com (ALussow) wrote:
>Can anyone tell me how many CD4 or CD8 molecules there 
are on the
>corresponding murine or human T-cell??  What is the 
surface area of the
>cell??
>
>Thanks 
>Alex
>If you don't ask much from life, that's what you'll get.
An interesting and difficult question.  Also a relatively 
useless
one as far as clinical applications.
At this time I can only propose to work around an answer. 
 The
cell diameter range that T cells far into is 9-12 microns. 
 Other
cells, such as immature basophils, also fall into this 
range.  If
one wants to assume a sphere that you can calculate from 
the
diameter.  Electronic cell counters measure volumne in
femtoliters.  This is true with RBCs.  I seem to remember 
seeing
somewhere that T cell volume is between 140 and 160 
femtoliters
(but need shape to calculate surface area), but I have no
reference to support this.  Just to be sure that we are on 
the
same page, I want memtion that one cannot classify a cell 
soley
volume.  Electronically derived WBC differentiatials use 
volume
as part of the classification.  The volume used however 
seems to
be found in the manufacture's software algrithm.  Such 
algrithms
are appearently company secrets.
As to the number of CD4 & CD8 receptors this would vary 
with cell
maturity and possibly with TCRs.  As TCRs are continuously
replaced so might CD4 & CD8.  I do not think that anyone 
knows
the answer to your question.  I would like to guess that 
it is in
the hundreds.  Someone out there correct me!



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