serum free media for hybridoma

Richard R. Hardy hardy at mighty.fccc.edu
Mon Nov 13 16:04:34 EST 1995


In article <dtyyu.106.00089808 at ucla.edu>, dtyyu at ucla.edu (David Tak Yan
Yu) wrote:

>I wish to culture hybridoma in serum free media to allow me to isolate the 
>antibody.  Can some one recommend a U.S. manufacturer?
>David Yu

We use HB101 serum-free (not protein-free) medium from Irvine Scientific
for making our antibodies.  You may need to passage your cells in mixtures
of serum-containing/serum-free medium for several passages to "wean" them
off serum.  However, many lines just grow fine directly in HB101. Good
luck!
____________________________________________________________________________
Richard R. Hardy                       Member, Institute for Cancer Research           
Fox Chase Cancer Center                Tel:    (215) 728-2463
7701 Burholme Ave.                     FAX:    (215) 728-2412
Philadelphia, PA 19111                 E-MAIL: R_HARDY at fccc.edu



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