DNA OD readings help

Theresa Cowan tcowan at morgan.ucs.mun.ca
Thu Nov 23 13:11:27 EST 1995


After PCR anplification, chloroform extraction and column purification.  
The 260/280 readings for my samples are between 2 and 4 (14 samples).  
I've asked around about this, only to get two different opinions.  1)  
The samples are very pure.  2)  The samples are contaminated. 
If I chloroform extract and ethanol/isopropanol extract my PCR products, 
the 260/280 readings are between 1.7 and 2.
Any suggestions?




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