Comet assay information

Dr. Manuel Saenz Terrazas a65820 at UACHIH.UACHNET.MX
Thu Nov 23 12:32:18 EST 1995


Hi everybody:
   We are working with induced apoptosis in thymus and spleen in mice and 
hamster. We would like to find detailed protocols to perform the comet 
assay. We are particularly interested in hearing comments regarding 
personal experience with this assay.
Is it suitable for thymus, spleen and even Peyer and lymph nodes?
Does it replace the apoptotic cell count with ethidium bromide/acridine 
orange and the ladder pattern in agarose DNA electrophoresis gel?
Some papers mention something about staining the gel with ethidium bromide. 
Does anybody has ever used acridine orange instead or in addition to 
Ethidium bromide?
Thanks in advance for any help.
                               Manuel Saenz M.D.

##########################################################################
Manuel Saenz M.D.                   INTERNET:a65820 at uachih.uachnet.mx
Department of Immunology            Personal Address:
School of Chemistry                     Degollado 3908
University of Chihuahua                 Departamento 2
Chihuahua, Chihuahua                    Col. Obrera
Mexico   CP 31110                       Chihuahua, Chihuahua
                                        Mexico   CP 31000
##########################################################################




More information about the Immuno mailing list