Immuno detection of T Cells ?

Richard Sanderson 101336.2567 at CompuServe.COM
Tue Nov 28 13:35:55 EST 1995

In reply to you problem with trying to detect cell surface 
antigen on you cells, you can try a number of methods.  You say 
you've tried ELISA but your cells don't stick to the plate...why 
bother?  Fix your cells with Azide and carry out a Fluorescence 
type ELISA on the whole cells.  Alternatively use a FACS anaylser 
or simply a UV microscope.

Hope that's of some help


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