Immuno detection of T Cells ?

Pierce Chemical Co PierceChem at mcimail.com
Mon Nov 27 15:43:56 EST 1995


One method of getting the cells to stick to the plates is to use a coated plate.  The cell could be 
biotinylated through the cell membrane proteins and then stuck to streptavidin coated plates.  
Also there are a variety of maleic anhydride coated plates that react directly with amine groups 
on the cell membranes to immobilize them.If either of these techniques is used, be sure to block 
any remaining reactive sites on the plates to prevent nonspecific binding.

Technical Assistance
Pierce Chemical Co
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E-mail: PierceChem at mcimail.com





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