Methods to make mAB fluorescent...
ANDREI.POPOV at bbsrc.ac.uk
Sun Oct 1 07:53:09 EST 1995
>i need help attaching a fluorescent marker on an monoclonal antibody. do
>you have any experience with such a procedure? if yes...please send the
>methodology. is there a kit available? if so...send the phone number and
just incubate your mABs with FITC, at molar ratio 1:2 or 1:3 in
Na carbonate buffer pH 9.3-9.6 for a couple of hours at RT (protect from
o/n at 4C with gentle stirring. Purify on Sepharose from unattached FITC
and that is it.
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