Methods to make mAB fluorescent...

Andrei Popov ANDREI.POPOV at
Sun Oct 1 07:53:09 EST 1995

>i need help attaching a fluorescent marker on an monoclonal antibody.  do
>you have any experience with such a procedure? if yes...please send the
>methodology.  is there a kit available? if so...send the phone number and
just incubate your mABs with FITC, at molar ratio  1:2 or 1:3 in
Na carbonate buffer pH 9.3-9.6 for a couple of hours at RT (protect from
light!) or
o/n at 4C with gentle stirring. Purify on Sepharose from unattached  FITC
and that is it.



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