Fragmentation of mouse IgG

Morgan Donaghy donaghym at agresearch.cri.nz
Wed Oct 11 10:05:28 EST 1995


>Has anyone got references for using ficin and cysteine to get Fab' 
>fragments?

>Ta very much (-:
Try Current Protocols in Immunuology, there is several variations on a method 
for papain digestion of IgG.
Cheers
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