I am naively attempting to perform an HIV p24 dot blot, with limited
success. For now, I am using lysates from cell cultures infected with
HIVNL4-3. I cannot achieve a uniform dot -- rather, I typically see a
halo\ring pattern. Otherwise, I can distinguish between positive and
negative lysates. I am using a biorad apparatus according to thier
instructions, and I am interested in techniques that might eliminate
my unusual blotting pattern. In particular, I wonder whether a larger
pore size might help (I am using 0.2micron). Thanks in advance for
any helpful hints.
John Ferbas, Ph.D.
UCLA
