Fragmentation of mouse IgG

Markus Macht macht
Thu Oct 12 02:23:32 EST 1995


Generating Fab' from F(ab')2 by cystein reduction is described in Meth. Enzym.
but i don't know the exact volume. The procedure works fine, i tried it with
two different murine monoclonal IgG1 antibodies an both gave Fab' fragments in
good yields. Products were detected with SDS-Page and MALDI-MS and looked fine.

Yours sincerely

Marcus Macht
University of Constance
78434 Constance
Germany




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