Our lab is trying to detect oncogene message expression using PCR in
human bone marrow progenitor cells and peripheral blood
mononucleocytes. We recently observed that the presence of human
red blood cells may interfere with the detection/expression of mRNA
in nucleated cells. We are wondering if there is anyone has
encountered similar situations? It would be greatly appreciated if
anybody could suggest which components from red blood cell may
contribute this phenomena. Many thanks.
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J. Bao, Ph.D.
Division of Allergy, Immunology
and Rheumatology Voice: 504 568-3074 (O)
Department of Pediatrics : 504 454-0653 (H)
LSU Medical Center Fax : 504 568-7532
Sch. of Med. in New Orleans
1542 Tulane Avenue Email: jbao at nomvs.lsumc.edu
New Orleans, LA 70112 jbao at mailhost.tcs.tulane.edu
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