Non radioactive cytotoxic activity assay

David Chia dchia at ucla.edu
Wed Oct 18 17:31:22 EST 1995


bioskill at gate.net (Biology Skills Center) wrote:
>I would like to set up a non radioactive cytotoxic activity assay for T 
>lymphocytes. Does anybody know a method (obviously not the traditional 
>chromium release assay) that is sensitive enough? Are there companies selling 
>some kit? I have access to a FACScan.
>
>Thanks for any advice.
>Pierre-Andre Chavaillaz

We have developed a non-radioactive cytotoxicity assay based on Calcein AM release. 
It was published in Human Immunology 37, 264-270, (1993). Hope it will be of help.

David Chia




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