Non radioactive cytotoxic activity assay

Jeffrey Frelinger jfrelin at MED.UNC.EDU
Wed Oct 18 14:41:04 EST 1995

There are a coupleof assays that measure induction of apoptosis using BrDU
staining, or PI staining and sfhits to less than 2N DNA content.  They are
not as convenient or sensitive.
Jeff Frelinger
At 05:25 PM 10/18/95 +0000, Biology Skills Center wrote:
>I would like to set up a non radioactive cytotoxic activity assay for T 
>lymphocytes. Does anybody know a method (obviously not the traditional 
>chromium release assay) that is sensitive enough? Are there companies selling 
>some kit? I have access to a FACScan.
>Thanks for any advice.
>Pierre-Andre Chavaillaz

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