Digitonin

[Ian A. York]

I'd like to lyse cells with digitonin, but I'm not clear on the 
preparation of the digitonin itself.  I note that some people use Aldrich 
digitonin, and go through a fairly elaborate preparation (either 
recrystallization, or preparation one week in advance followed by letting 
it sit and filtering out crystals, I think).  Others don't make any 
comments about special preparation.  I note that Sigma sells two forms of 
digitonin - one is noted 'for aqueous preparations'.  

Does anyone have experience with digitonin?  Comments on preparation, 
suppliers, use?

Thanks for any help.

Ian

-- 
Ian York   (york at mbcrr.harvard.edu)
Dana-Farber Cancer Institute, 44 Binney St., Boston MA 02115
Phone (617)-632-3921     Fax  (617)-632-2627