Ian A. York
york at mbcrr.dfci.harvard.edu
Wed Oct 18 11:25:57 EST 1995
I'd like to lyse cells with digitonin, but I'm not clear on the
preparation of the digitonin itself. I note that some people use Aldrich
digitonin, and go through a fairly elaborate preparation (either
recrystallization, or preparation one week in advance followed by letting
it sit and filtering out crystals, I think). Others don't make any
comments about special preparation. I note that Sigma sells two forms of
digitonin - one is noted 'for aqueous preparations'.
Does anyone have experience with digitonin? Comments on preparation,
Thanks for any help.
Ian York (york at mbcrr.harvard.edu)
Dana-Farber Cancer Institute, 44 Binney St., Boston MA 02115
Phone (617)-632-3921 Fax (617)-632-2627
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