Non radioactive cytotoxic activity assay

David Chia dchia at
Wed Oct 18 17:48:04 EST 1995

bioskill at (Biology Skills Center) wrote:
>I would like to set up a non radioactive cytotoxic activity assay for T 
>lymphocytes. Does anybody know a method (obviously not the traditional 
>chromium release assay) that is sensitive enough? Are there companies selling 
>some kit? I have access to a FACScan.
>Thanks for any advice.
>Pierre-Andre Chavaillaz

We have developed a non-radioactive cytotoxic assay based on Cacein AM release, and 
the method was published in Human Immunology 37, 264 (1993). Hope it will be of 

David Chia

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