Non radioactive cytotoxic activity assay

[David Chia]

bioskill at gate.net (Biology Skills Center) wrote:
>I would like to set up a non radioactive cytotoxic activity assay for T 
>lymphocytes. Does anybody know a method (obviously not the traditional 
>chromium release assay) that is sensitive enough? Are there companies selling 
>some kit? I have access to a FACScan.
>
>Thanks for any advice.
>Pierre-Andre Chavaillaz

We have developed a non-radioactive cytotoxic assay based on Cacein AM release, and 
the method was published in Human Immunology 37, 264 (1993). Hope it will be of 
help.

David Chia