Non radioactive cytotoxic activity assay

David Chia dchia at ucla.edu
Wed Oct 18 17:48:04 EST 1995


bioskill at gate.net (Biology Skills Center) wrote:
>I would like to set up a non radioactive cytotoxic activity assay for T 
>lymphocytes. Does anybody know a method (obviously not the traditional 
>chromium release assay) that is sensitive enough? Are there companies selling 
>some kit? I have access to a FACScan.
>
>Thanks for any advice.
>Pierre-Andre Chavaillaz

We have developed a non-radioactive cytotoxic assay based on Cacein AM release, and 
the method was published in Human Immunology 37, 264 (1993). Hope it will be of 
help.

David Chia




More information about the Immuno mailing list