Non radioactive cytotoxic activity assay
dchia at ucla.edu
Wed Oct 18 17:48:04 EST 1995
bioskill at gate.net (Biology Skills Center) wrote:
>I would like to set up a non radioactive cytotoxic activity assay for T
>lymphocytes. Does anybody know a method (obviously not the traditional
>chromium release assay) that is sensitive enough? Are there companies selling
>some kit? I have access to a FACScan.
>Thanks for any advice.
We have developed a non-radioactive cytotoxic assay based on Cacein AM release, and
the method was published in Human Immunology 37, 264 (1993). Hope it will be of
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