Isolation of type II alveolar epithelial cells

Juliet Palethorpe juliet at
Fri Oct 20 02:35:14 EST 1995

Can anyone offer any advice regarding isolation of type II alveolar
epithelial cells from the rat lung?

I have been attempting to follow the method of Dobbs and others (1986) but
have experienced major difficulties.

I have tried using Elastase from both Sigma and Worthington and delivery
of the enzyme to the lung via the trachea or after chopping the whole lung
but can only liberate a very low number of cells and a low percentage of
type II cells (30%) in this inital crude cell suspension.

Can anyone describe in detail the conditions required for successful
panning on IgG coated plates?

Any suggestions would be much appreciated.

Juliet Palethorpe

TVW Telethon Institute for Child Health Research
(Company Limited by Guarantee)
Western Australia

Fax 61 9 388 3414

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