Hi all,
does anybody out there have experience of class switching M
hybridomas to G. I don't have access to a FACS for sorting, so it
would have to be done manually by cloning. I have heard of people
doing it by this method and wondered how feasible it really was.
Also I know that highly specific isotyping reagents would be needed
to identify the class-switched clones. Many of the reagents we have
looked at across the years give some cross reactivity with some
other isotypes, does anybody know of a good source?
Thanks
Rob
Robert Burns
Monoclonal Antibody Unit
Scottish Agricultural Science Agency
East Craigs
Edinburgh
Scotland
burns at sasa.gov.uk
