Phenotyping mouse brain cells in situ

[walker at mbcf.stjude.org]

We are immunophenotyping normal mouse brain cells by 
flow and direct staining of frozen and fixed brain
tissue.  We can easily detect certain phenotypes by
flow but are unable to detect such cells in fixed or
frozen sections.  One possibility is that cells in
the sections are 'coated' with substances that are
removed during preparation of the cell suspensions.
Any one have a similar experience?  Any solution to
in situ detection?  All suggestions appreciated. 
Thanks.



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William S. Walker, Ph.D.
Department of Immunology
St. Jude Children's Research Hospital
Memphis, Tennessee USA 38105-2794
bill.walker at stjude.org
Voice: (901) 495-2537 
FAX: (901) 495-3107
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