class switching M hybridomas
Richard R. Hardy
hardy at mighty.fccc.edu
Fri Oct 27 10:46:17 EST 1995
In article <MAPI.Id.0016.0075726e732020203341464630303033 at MAPI.to.RFC822>,
burns at sasa.gov.uk (Robert Burns) wrote:
> does anybody out there have experience of class switching M
>hybridomas to G. I don't have access to a FACS for sorting, so it
>would have to be done manually by cloning. I have heard of people
>doing it by this method and wondered how feasible it really was.
>Also I know that highly specific isotyping reagents would be needed
>to identify the class-switched clones. Many of the reagents we have
>looked at across the years give some cross reactivity with some
>other isotypes, does anybody know of a good source?
I've done this by FACS, not sure how well it would work by limit diln (our
estimates of frequency varied from 1/10^6 to 1/10^5). As to source of
anti-isotype reagents, you could try PharMingen (San Diego) - I think they
have monoclonal anti-isotype reagents. Good luck!
Richard R. Hardy Member, Institute for Cancer Research
Fox Chase Cancer Center Tel: (215) 728-2463
7701 Burholme Ave. FAX: (215) 728-2412
Philadelphia, PA 19111 E-MAIL: R_HARDY at fccc.edu
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