Peptide conjugation efficiency
George A. Heavner
gheavner at omni.voicenet.com
Thu Oct 26 16:10:38 EST 1995
Amino acid analysis can be used to determine the degree of peptide conjugation
to a carrier protein. The best numbers can be obtained if you incorporate an
unnatural amino acid (e.g. Nle) into the peptide. This makes determination of
coupling efficiency easy. If the coupling technique covalently modifies an
amino acid with a non-hydrolyzable linkage (e.g. addition to a cysteine), this
modified amino acid can be used in quantitation. Incorporation of a
chromophore with a unique absorption wavelength could also be used in
George A. Heavner, Ph.D.
Senior Director, Peptide R&D
In article <46mju1$ock at ixnews7.ix.netcom.com> nancied at ix.netcom.com (Nanci E
Donacki ) writes:>From: nancied at ix.netcom.com (Nanci E Donacki )
>Subject: Peptide conjugation efficiency
>Date: 26 Oct 1995 00:14:57 GMT
>Does any one know of a procedure to determine the efficiency of a
>peptide coupled to a protein carrier? The only method I know of
>requires radiolabeled peptides, and then these can't be used for
>immunization of animals.
>Are there any other procedures that can be used?
>Thanks in advance.
>Nanci E Donacki
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