Non radioactive cytotoxic activity assay
Nikolaos G. Papadopoulos
ngpdoc at athena.compulink.gr
Sun Oct 29 17:35:47 EST 1995
In article <9510181941.AA00537 at charlotte.med.unc.edu>,
jfrelin at MED.UNC.EDU says...
>There are a coupleof assays that measure induction of apoptosis using
>staining, or PI staining and sfhits to less than 2N DNA content. They
>not as convenient or sensitive.
>At 05:25 PM 10/18/95 +0000, Biology Skills Center wrote:
>>I would like to set up a non radioactive cytotoxic activity assay for T
>>lymphocytes. Does anybody know a method (obviously not the traditional
>>chromium release assay) that is sensitive enough? Are there companies
>>some kit? I have access to a FACScan.
>>Thanks for any advice.
Try J.Imm.Meth. (1994) 177:101-111 for a cytotoxity method,using a
FACScan and fluorochromes easily obtainable from Molecular probes.
It is more sensitive than Cr-release and definitely more convenient if
one cultures cell-lines on a routine basis
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