Purification of F(ab)2 from hybridoma

[Peter Kwasowski]

On 28 Oct 1995 23:20:47 GMT, you wrote:

>In article <46qntd$4v5e at tigger.cc.uic.edu>
>Keld Sorensen <KeldS at uic.edu> writes:

>> How about an antibody agains Fab2 immobilized on Sepharose?
>> Keld.

>We use this system, normally, but run into the problem that the light
>chain comes off with the whole Fab2.

>Jake


OK!

So how about adsorbing the anti-Fab2 antibodies with light chains
before immobilising them?

Pete