PHA stimulation of T cells

Chunlei Liu Chunlei.Liu at dartmouth.edu
Fri Sep 1 08:12:51 EST 1995


I am trying to use PHA and IL-2 stimulation to maintain human T cell
clones for long term.  The trouble I have is that feeder cells alone
show robust proliferation when treated with 5ug/ml PHA and 20U/ml IL-2.
 The feeder cells are 3300 rad irradiated human peripheral blood
mononuclear cells (PBMC). Anybody has an idea how to modify the
conditions of stimulation and therefore eliminate the proliferation of
feeder cells without interfering accessory function?  Appreciated.



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