Mycoplasma comtamination of hybridoma?

David L. Haviland, Ph.D. HAVILAND at KIDS.WUSTL.EDU
Fri Sep 1 17:23:34 EST 1995


In <424pvk$g9p at news1.best.com> user at alexon.com writes:

>    I'm seeking info on characteristics, diagnostsis, and treatment of 
> mycoplasma contamination in hybridoma cell lines. I have suspected 
> contamination in one of my cell lines since I have had recurrent 
> problems with solid tumor production and continually recieve "bloody" 
> ascites. The cells, however, grow at a satisfactory rate and there has 
> been no drop in MAb performance.
>    Suggestions of products and/or protocol would be appriecated.


Yes... I'd like to think I can help.

We use the Stratagene Mycoplasma PCR detection with good results.  The 
primer annealing may need adjustment(s) per the machine being used.  As a 
backup, we sometimes use the Gibco BRL detection kit that employs the 3T6 
murine cell line but this kit takes nearly 5-6 days before you know the 
answer.  Ergo, first choice is the St_Gene assay.

As far as treatement goes, we have had good success with the BM_Cycline 
treatment kit and if memory serves, it comes from BMB.

I have no affiliation with any of the three companies listed above.

Like my friend, Jim Graham, I abhore kits, but these do work at least in 
our hands.

Hope this helps,
David




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