PHA stimulation of T cells

Bronwyn Venus venusb
Wed Sep 6 20:29:01 EST 1995

On 1 Sep 1995, Chunlei Liu wrote:

> I am trying to use PHA and IL-2 stimulation to maintain human T cell
> clones for long term.  The trouble I have is that feeder cells alone
> show robust proliferation when treated with 5ug/ml PHA and 20U/ml IL-2.
>  The feeder cells are 3300 rad irradiated human peripheral blood
> mononuclear cells (PBMC). Anybody has an idea how to modify the
> conditions of stimulation and therefore eliminate the proliferation of
> feeder cells without interfering accessory function?  Appreciated.

Please excuse my ignorance but why are you using feeder cells?
My collegue is currently growing tcell clones with no feeders.

Bronwyn Venus
Animal Research Institute
665 Fairfield Road
Yeerongpilly QLD 4105

E-mail: venusb at

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