M Clark re IgM purif.

Dr. Mike Clark mrc7 at cam.ac.uk
Mon Sep 11 11:18:21 EST 1995

In article <preston-0809951636370001 at> , Mike Preston
<mailto:preston at warren.med.harvard.edu> wrote:
> Mike,
> When you preciptitate murine IgM in low salt buffers or water, do they
> redissolve readily in PBS and do they generally reatin their activity?
> Your expertise is appreciated.
> Mike Preston
My experience with euglobulin precipitation is that some monoclonals do
lose their activities but others don't! So the best thing is to try it on
a sample of the antibody before committing a whole batch.
Also for redissolving I sometimes found that PBS at pH7.2 didn't work well
but that bicarbonate buffered saline at ph8.0 did. I was using bicarbonate
buffers to do my labeling with biotinyl-succinimide ester.

Incidently, the rat IgG2c subclass also behaves as a euglobulin.

Mike Clark, mrc7 at cam.ac.uk          http://www.path.cam.ac.uk/~mrc7/
  o/ \\    //            ||  ,_ o   Dr. M.R. Clark, Division of Immunology
 <\__,\\  //   __o       || /  /\,  Cambridge University, Dept. Pathology
  ">    ||   _`\<,_    //  \\ \> |  Tennis Court Rd., Cambridge CB2 1QP
   `    ||  (_)/ (_)  //    \\ \_   Tel. 01223 333705  Fax. 01223 333875

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