M Clark re IgM purif.

[Dr. Mike Clark]

In article <preston-0809951636370001 at 134.174.147.63> , Mike Preston
<mailto:preston at warren.med.harvard.edu> wrote:
> 
> Mike,
> When you preciptitate murine IgM in low salt buffers or water, do they
> redissolve readily in PBS and do they generally reatin their activity?
> Your expertise is appreciated.
> 
> Mike Preston
> 
My experience with euglobulin precipitation is that some monoclonals do
lose their activities but others don't! So the best thing is to try it on
a sample of the antibody before committing a whole batch.
Also for redissolving I sometimes found that PBS at pH7.2 didn't work well
but that bicarbonate buffered saline at ph8.0 did. I was using bicarbonate
buffers to do my labeling with biotinyl-succinimide ester.

Incidently, the rat IgG2c subclass also behaves as a euglobulin.


Mike Clark, mrc7 at cam.ac.uk          http://www.path.cam.ac.uk/~mrc7/
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