Assay protein bound to ELISA
Geoffrey D. Wheelock
74710.2416 at CompuServe.COM
Mon Sep 11 20:47:17 EST 1995
Someone asked how to assay protein bound to immulon 2 ELISA plates.
My reader trashed the original article so I am replying from memory.
The amount of protein bound to the plate will be very small, circa
100 ng or less, so you need a really sensitive assay. I have done
it by iodinating the protein or peptide, determining the specific
activity of the protein, binding to the plate, washing, and counting
the wells in a gamma counter. Of course, if you are not using strip
wells, breaking up the plate into individual wells is problematic :)
Hope this helps. Geoffrey D. Wheelock
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