small volume dialysis

Mark Kaplan mkaplan at mbcrr.harvard.edu
Wed Sep 13 12:23:19 EST 1995


In article <ctrambas-1309951228300001 at mg1_56.med.utas.edu.au>,
ctrambas at postoffice.utas.edu.au wrote:

> Does anybody know of an easy and reliable method for dialysis of very
> small volumes? (eg. for removing azide from commercial antibody sources)
> Would appreciate any input. Thankyou.

   Put your sample in a microfuge tube.  Poke a hole or two in the top of
the tube with an 18 ga. needle.  Place appropriate dialysis membrane over
the opening of the tube and close the top.  Place the tube in a floater
and put the entire unit inverted into the buffer you want to dialyze
against.  Only cautionary note is to be sure the tubing doesn't rip when
you close the top.

                                                   Mark Kaplan



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