Staining tissue for Macrophages
hoekstra at unixg.ubc.ca
Wed Sep 13 22:43:48 EST 1995
I have suspected xanthoma lesions (bird) which should contain macrophages
and multi-nucleated giant cells if a TRUE xanthoma. Suggestions for
staining 6 micron thick sections (cryostat).
1.Should I fix the sections first? What to use?
2.Is the cold temperature storage destroyed by WBC's etc. (i.e. -70 C)
3.What staining to use on the tissue? Tried Wrights, Hematoxyolin, Giesma
etc. with no luck.
More information about the Immuno