Staining tissue for Macrophages

Sucking Gippy hoekstra at
Wed Sep 13 22:43:48 EST 1995

I have suspected xanthoma lesions (bird) which should contain macrophages 
and multi-nucleated giant cells if a TRUE xanthoma. Suggestions for 
staining 6 micron thick sections (cryostat).

1.Should I fix the sections first? What to use?

2.Is the cold temperature storage destroyed by WBC's etc. (i.e. -70 C)

3.What staining to use on the tissue? Tried Wrights, Hematoxyolin, Giesma 
etc. with no luck.

Thanks. SG

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