cloning IgG

Greg Tobin tobin at fcs260c.ncifcrf.gov
Mon Sep 11 08:18:04 EST 1995


I'd like a little advice in cloning an IgG molecule from the
N-terminus to the joint.  The sequence is not known so I would like
to use degenerate oligos to amplify cDNA from a human hybridoma.
Can anyone give me a reference (or the sequences) for 5' oligos
in the signal sequences of the heavy and light chains and for 
3' oligos at the end of light and at the joint of heavy?

Thanks very much.
-Greg






-- 
Greg Tobin, Ph.D.                        tobin at lcms-1.ncifcrf.gov




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