Immunoprecipitation

Ken Frauwirth BioKen frauwirt at bacillus.Berkeley.EDU
Mon Apr 1 13:44:02 EST 1996


In article <Pine.OSF.3.91.960401131637.23103A-100000 at sable.ox.ac.uk>,
Al Reavley  <uzdb0017 at sable.ox.ac.uk> wrote:
>I hope someone can answer the following question for me:-
>
>Are all antibodies suitable for immunoprecipitation, and if not why not?
>


Not all antibodies are usable for immunoprecipitation.  Antibodies raised
against peptides or denatured proteins will recognize linear epitopes that
may be hidden in the native protein, which is the usual target for an
immunoprecipitation.  Even antibodies raised against purified native protein
may not work for IP if the antibody epitope is hidden by interaction with 
another protein or cofactor.  The only way to know is to test each antibody.  
Generally, however, an antibody that works for other methods that detect 
native protein (ELISA, flow cytometry, etc.) should work for IP as well.

BioKen
-- 
Ken Frauwirth (MiSTie #33025)                         _           _
frauwirt at mendel.berkeley.edu                         |_) *    |/ (_ |\ |
http://mendel.berkeley.edu/~frauwirt/HomePage/       |_) | () |\ (_ | \|  
DNRC Title: Chairman of Joint Commission on In-duh-vidual Affairs



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