I just thought I'd do my share to improve the recently-abysmal
signal-to-noise ratio of this newsgroup...
I'm studying the immunology of class I MHC in owl monkeys.
Nothing has yet been published about owl monkey CTL biology, nor is the
sequence of owl monkey Il-2 known. However, a study (Smith S et. al, Ann.
N.Y. Acad. Sci. 653:146 ) has shown that most anti-human leukocyte
MAb's do not react with owl monkey PBMC's. Thus I expect that my odds of
getting human Il-2 to work in this system will be low.
So, how would I go about cloning and propagating antigen-specific
CTL's? In the bad old days before Il-2, did people use irradiated feeder
cells? Would PBMC feeders suffice? Sacrificing an animal and isolating
splenocytes would be out of the question for my experiment. I want to
accomplish this with bleeding only.
Your advice and pointers to relevant references are greatly
Unique ID : Ladasky, John Joseph Jr.
Title : BA Biochemistry, U.C. Berkeley, 1989 (Ph.D. perhaps 1998???)
Location : Stanford University, Dept. of Structural Biology, Fairchild D-105
Keywords : immunology, music, running, Green