Cloning CTL's in New Species - How?

John Ladasky ladasky at leland.Stanford.EDU
Sun Apr 7 01:32:19 EST 1996

Greetings, everyone,

	I just thought I'd do my share to improve the recently-abysmal
signal-to-noise ratio of this newsgroup...

	I'm studying the immunology of class I MHC in owl monkeys.
Nothing has yet been published about owl monkey CTL biology, nor is the
sequence of owl monkey Il-2 known.  However, a study (Smith S et. al, Ann.
N.Y. Acad. Sci. 653:146 [1992]) has shown that most anti-human leukocyte
MAb's do not react with owl monkey PBMC's.  Thus I expect that my odds of
getting human Il-2 to work in this system will be low.

	So, how would I go about cloning and propagating antigen-specific
CTL's?  In the bad old days before Il-2, did people use irradiated feeder
cells?  Would PBMC feeders suffice?  Sacrificing an animal and isolating
splenocytes would be out of the question for my experiment.  I want to
accomplish this with bleeding only.

	Your advice and pointers to relevant references are greatly

Unique ID : Ladasky, John Joseph Jr.
Title     : BA Biochemistry, U.C. Berkeley, 1989  (Ph.D. perhaps 1998???)
Location  : Stanford University, Dept. of Structural Biology, Fairchild D-105
Keywords  : immunology, music, running, Green

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