Need help doing Immuno-Magnetic Separation

[wick at cris.com]

I am just getting starting using an  Immuno-Magnetic Separation (IMS) technique for isolation of organisms from 
soil and environmental water samples. My purpose is to isolate the organism (crypto, giardia, microsporidia, etc.), 
extract the DNA and amplify to determine if the organism is present or not in the test samples. It is known that 
common water and soil samples contain inhibitors to PCR, so this technique, in theory, seems like a solution 
to this problem. I would like to exchange ideas with anyone with experience using IMS to solve the inhibitor 
problem.

Jim Wick

		Molecular Biology Resources, Inc.
E-mail:		wick at cris.com		
Phone:		414-871-7199
FAX:		414-871-1273
Snail Mail:	5520 W. Burleigh St.
		Milwaukee, WI 53210

"Don't precipitate more than you can resuspend"