Quantitative cytokine bioassay

David Laughland gbza83 at udcf.gla.ac.uk
Wed Apr 10 06:58:36 EST 1996


In article <4j5ins$lki at mserv1.dl.ac.uk>, "Zeitlin, Ben" <B.Zeitlin at shu.ac.uk> says:
>
>
>Could anyone point me towards any references for currently used bioassays 
>measuring IL-1 (beta), IL-2 and IL-6 levels in cell culture supernatant. 
>  And are there any bioassays for TNF alpha other than the WEHI or L929(?) 
>cytotoxicity assays.   To put it bluntly, I'm looking for methods which are 
>cheaper than ELISA!
>
>Thanks for your help,
>
I'm not sure that biassays would be any cheaper than ELISA, but 
'Cytokines a practicle Approach' has several methods which might interest you.
Janet Spicer



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