Native Gel Difficulty

nc26 at wwa.com nc26 at wwa.com
Fri Apr 12 19:20:39 EST 1996

Can someone help me with the following problem?  I am trying to run a western blot against
surface protein on a native gel.  The protein is not reactive with the antibody in a reduce form,
thus the native gel.  The problem is the surface protein will not migrate into the gel in its native
form.  I stain the gel with Coomassie Blue and clearly see the protein getting stuck in the 4%
stacker.  My sample buffer contains 3.125% SDS in a 0.1 M Tris buffer.  Does anyone know of a
protocol that would allow me to keep my protein in its native form on an SDS-PAGE gel?  Thanks
in advance for any information.

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