5 minute Murine In Vivo Adoptive transfer assay

rjjensn at aol.com rjjensn at aol.com
Thu Apr 25 23:27:14 EST 1996

I have been having trouble with Flurochrome labeling of Mouse LN 
cells. All labels downregulate L-selectin (CD62-L)  except Hoechst, 
and our flow cytometer is unable to handle UV. The tags Ive tried are 
TRIRC, DiI, CMFDA, Pkh-26, and LDS If anyone can help with a dye that 
doesnt effect homing to LN I would be grateful.
rjjensn at avalon.net

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