I have been having trouble with Flurochrome labeling of Mouse LN
cells. All labels downregulate L-selectin (CD62-L) except Hoechst,
and our flow cytometer is unable to handle UV. The tags Ive tried are
TRIRC, DiI, CMFDA, Pkh-26, and LDS If anyone can help with a dye that
doesnt effect homing to LN I would be grateful.
rjjensn at avalon.net