Cheng-Prusoff Equation: Upgrades Un

Dmitry Yuryev yur77 at glas.apc.org
Sat Aug 10 02:55:16 EST 1996


   Note my a paper which proves that analysis of inhibition
curves with formally exact computer programs can be performed only 
if experiment is conducted under "Cheng-Prusoff conditions".
   The summary is appended below. Full text (MS Word 6.0 for Win format)
is available from: ftp://garbo.uwasa.fi/pc/science/crsaf_02.zip

-----------------------


   REFINING CHENG-PRUSOFF EQUATION

   Dmitriy K. Yuryev   
   yur77 at glas.apc.org
   http://www.glasnet.ru/~yur77

   A generalisation of the Cheng-Prusoff formula relating affinity
constant and the concentration of inhibitor giving 50% inhibition has
been derived for the case when concentrations of ligands are not in
a great excess. It shows that methods ensuring precise solving of
binding equations (including computer approaches and equations derived
in the present work) can not be used if these "Cheng-Prusoff
conditions" of ligands' excess are not observed. The trouble is that
beyond these conditions analysis becomes unreliable: the error
contained in estimations of necessary parameters appears abruptly
multiplied in the calculated affinity constant



More information about the Immuno mailing list