Help! CD3+ Flow Cytometry

Dave Peritt peritt at mail.med.upenn.edu
Mon Aug 12 06:58:25 EST 1996


In article <4uitdo$nj7 at newsbf02.news.aol.com> SNOWTREK, snowtrek at aol.com
writes:
>First 2 responses: Yeah, but my purpose is a standardized, quantitatively
>well-characterized assessment of this anti-CD3 mab's binding. I need to
be
>able to walk over to the incubator and pull out a T-175  full of cell
XYZ,

actually CD3 levels on PB T cells is a very narrow window.  I think the
donor variability is far less than cell lines in culture.  Seperation of
blood is not that difficult but you need a donor source.....



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