Help! CD3+ Flow Cytometry

Mike Clark mrc7 at
Mon Aug 12 05:41:16 EST 1996

In article <4uitdo$nj7 at>, SNOWTREK
<mailto:snowtrek at> wrote:
> First 2 responses: Yeah, but my purpose is a standardized, quantitatively
> well-characterized assessment of this anti-CD3 mab's binding. I need to be
> able to walk over to the incubator and pull out a T-175  full of cell XYZ,
> grab a quarter million, mix in the mab, and get reasonable reproducibility
> in response. The variability and poor characterization of PBLs (possible
> pathogen present?, etc) makes them a non-viable alternative. Third
> response: The Jurkats didn't work for me. As you suggest, recloning them
> might allow isolation of a CD3 dense, homogeneous poulation. And, I'll
> look into the Molt16s. THANKS for the replies!!!!
Very interesting, do I detect from this request that we may be seeing
a replacement for that well used mouse antibody OKT3 appearing soon?
There are of course several humanised CD3 antibodies around including
humanised OKT-3 which has been sitting in the freezer since Celltech
finished it several years ago.

Mike Clark, mrc7 at
  o/ \\    //            ||  ,_ o   Dr. M.R. Clark, Division of Immunology
 <\__,\\  //   __o       || /  /\,  Cambridge University, Dept. Pathology
  ">    ||   _`\<,_    //  \\ \> |  Tennis Court Rd., Cambridge CB2 1QP
   `    ||  (_)/ (_)  //    \\ \_   Tel. 01223 333705  Fax. 01223 333875

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