Immunohistochemistry using anti-CD28 Ab

cdong at PRL.PULMONARY.UBC.CA cdong at PRL.PULMONARY.UBC.CA
Mon Aug 12 18:22:51 EST 1996


Hello there:

I have been trying to stain formalin-fixed and paraffin-embedded tissue 
specimens with anti-CD28 Ab.  I obtained the Ab from CALTAG 
Laboratories, Burlingame, CA and tried different antigen retrieval 
methods, and even used in flash-frozen tissues.  The antibody, 
however, never worked.  The same thing happened to Abs against CD80 
(B7.1) and CD86 (B7.2).  Does anyone have any experience in this 
regard or know of a source of Abs which may work for 
immunohistochemistry?  Any information would be greatly appreciated!

Chunming



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