Double labelling immunohist. with 2 mouse ab.s

SNOWTREK snowtrek at aol.com
Wed Aug 14 23:20:15 EST 1996


* [assuming the antibodies are monoclonal]Are the 2 mouse mabs of the same
isotype? One kappa, one lambda would help your cause.
* rabbit anti-mouse what? gamma?  
* how about doing FITC and RPE or Rhodamine conjugations of the 2 mouse
mabs?
* Here's something I've never done; preincubate one of the mouse mabs with
the FITC anti-mouse, the other of the mouse mabs with the rhodamine
anti-mouse, isolate the antibody::antibody complexes on a sizing column,
then add to your tissue sample. Wow, sounds like a mess.



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