HELP!!! Fungal contamination of IMPORTANT transfectants!

Kiley R. Prilliman kprillim at rex.re.uokhsc.edu
Sat Aug 17 22:29:21 EST 1996


To anyone who can help me:

The past four months of my work is hanging in the balance as I write
this, so ANY responses will be met with great enthusiasm!

It appears that some transfectants which I have been slaving away to
make have become fungally contaminated during their lengthy incubation
under G418 selection in my CO2 incubator.  Is there ANYTHING I can do to
save these cells ("clean them up"), or are my next 3-4 months to be
spent remaking all of my transfectants?

THANK YOU FOR YOUR HELP!

Kiley R. Prilliman
University of Oklahoma Health Sciences Center
Oklahoma City, OK



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