brett at BORCIM.WUSTL.EDU
Mon Aug 26 18:21:17 EST 1996
>Hi, out there...
>I remember hearing once that in mouse spleen cell proliferation assays, the
>stimulating antigens should not be dissolved in Tris buffers. Can anyone
>elaborate: is this true? Is there something about Tris buffers that is
>incompatible with lymphocyte proliferation assays? If so, can anyone
>explain what it is?
>Thanks for any advice!
>Lawrence S. D. Anthony, Ph.D.
>StressGen Biotechnologies Corp.
>120-4243 Glanford Avenue,
>Victoria, B.C. V8Z 4B9
>Web Page: http://www.stressgen.com/stressgen
I wouldn't touch any cell with a trizma buffered solution. It has a propensity
to buffer the endocytic pathway and no doubt gums up antigen processing in
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