Tris buffers

brett brett at BORCIM.WUSTL.EDU
Mon Aug 26 18:21:17 EST 1996


>Hi, out there...
>
>I remember hearing once that in mouse spleen cell proliferation assays, the
>stimulating antigens should not be dissolved in Tris buffers.  Can anyone
>elaborate: is this true?  Is there something about Tris buffers that is
>incompatible with lymphocyte proliferation assays?  If so, can anyone
>explain what it is?
>
>Thanks for any advice!
>
>Larry
>
>Lawrence S. D. Anthony, Ph.D.
>Senior Scientist/Immunology
>StressGen Biotechnologies Corp.
>120-4243 Glanford Avenue,
>Victoria, B.C.  V8Z 4B9
>Tel: 604-744-2811
>Fax: 604-744-2877
>Web Page: http://www.stressgen.com/stressgen

I wouldn't touch any cell with a trizma buffered solution. It has a propensity
to buffer the endocytic pathway and no doubt gums up antigen processing in
such assays.




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