CYTOKINE secretion - assay or PCR?
Wed Feb 7 18:53:18 EST 1996
On Mon, 5 Feb 1996 alena at scientia.up.ac.za wrote:
> I am kind of new in the field of immunology and have more experience in
> cloning. Part of my new project aims the quantitation of cytokine production
> by T cells. I was wondering what the easiest way is to do this. Is
> Quantitative PCR the best and reliable way to do this? Or does this type of
> PCR take a lot of time to optimize or is it just not possible to do? Or can
> one best do cytokine assays (like the CTLL-2 assay for IL-2 determination?
> Everyone who has experience in this area could really be of help to me! So
> please respond, thanks in advance!
> Anne Lenaerts (belgium)
> Department of Biochemistry
> Laboratory of Immunology
> South Africa
I have developed ELISA protocols to determine cytokine production.
Commercial assays are available, and save heaps of time.
I am using sheep cells, so must develope these things myself.
Let me now if you need help
Animal Research Institute
665 Fairfield Road
Yeerongpilly QLD 4105
E-mail: venusb at planet.mh.dpi.qld.gov.au
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