CYTOKINE secretion - assay or PCR?

Elliott Dawson bvepd at Telalink.Net
Wed Feb 7 20:24:15 EST 1996


If you are wanting to quantitate the cytokines, then Immunoassay is probably 
the most desireable.  Quantitative PCR is not as straight forward and to get 
meanigful data you would have to perform quantitative RT-PCR on the mRNA's 
for the cytokines which would introduce an entirely different set of 
kinetics regarding stability, 1/2 life etc for these RNA's.  It is far 
better to measure the cytokines themselves.  Amersham, R&D systems and 
Genzyme, to name but three, have very good cytokine assays available.




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